ATAD2 controls chromatin-bound HIRA turnover.
Life Sci Alliance
; 4(12)2021 12.
Article
en En
| MEDLINE
| ID: mdl-34580178
Taking advantage of the evolutionary conserved nature of ATAD2, we report here a series of parallel functional studies in human, mouse, and Schizosaccharomyces pombe to investigate ATAD2's conserved functions. In S. pombe, the deletion of ATAD2 ortholog, abo1, leads to a dramatic decrease in cell growth, with the appearance of suppressor clones recovering normal growth. The identification of the corresponding suppressor mutations revealed a strong genetic interaction between Abo1 and the histone chaperone HIRA. In human cancer cell lines and in mouse embryonic stem cells, we observed that the KO of ATAD2 leads to an accumulation of HIRA. A ChIP-seq mapping of nucleosome-bound HIRA and FACT in Atad2 KO mouse ES cells demonstrated that both chaperones are trapped on nucleosomes at the transcription start sites of active genes, resulting in the abnormal presence of a chaperone-bound nucleosome on the TSS-associated nucleosome-free regions. Overall, these data highlight an important layer of regulation of chromatin dynamics ensuring the turnover of histone-bound chaperones.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
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Factores de Transcripción
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Nucleosomas
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Transducción de Señal
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Proteínas de Ciclo Celular
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Proteínas de Schizosaccharomyces pombe
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Proteínas de Unión al ADN
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Chaperonas de Histonas
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Células Madre Embrionarias de Ratones
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ATPasas Asociadas con Actividades Celulares Diversas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Life Sci Alliance
Año:
2021
Tipo del documento:
Article