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Morphofunctional Properties of Corneal Stromal Cells.
Surovtseva, M A; Poveshchenko, O V; Krasner, K Yu; Kim, I I; Lykov, A P; Bondarenko, N A; Shul'mina, L A; Trunov, A N; Chernykh, V V.
Afiliación
  • Surovtseva MA; Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. mfelde@ngs.ru.
  • Poveshchenko OV; Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Krasner KY; S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health, Novosibirsk, Russia.
  • Kim II; Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Lykov AP; Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Bondarenko NA; Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Shul'mina LA; Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Trunov AN; S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health, Novosibirsk, Russia.
  • Chernykh VV; S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health, Novosibirsk, Russia.
Bull Exp Biol Med ; 172(1): 96-99, 2021 Nov.
Article en En | MEDLINE | ID: mdl-34791562
ABSTRACT
Human corneal stromal cells were isolated by enzymatic digestion from a new source, lenticules obtained during laser vision correction by the ReLEx SMILe method. The resulting culture was mainly presented by fibroblast-like cells with a phenotype CD90-/CD73+/CD105+/keratocan-/lumican-/ALDH1A1+ that differentiate into keratocytes in a specialized medium. The concentration of fetal calf serum-derived growth factors affects the rate of proliferation, production of erythropoietin and brain neurotrophic factor by corneal fibroblasts, and to a lesser extent, their migration activity and production of extracellular matrix components. Thus, the high functional potential of fibroblast-like cells isolated from stromal lenticles can be used to develop cell technologies in ophthalmology.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Células del Estroma / Sustancia Propia / Queratocitos de la Córnea / Fibroblastos Idioma: En Revista: Bull Exp Biol Med Año: 2021 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Células del Estroma / Sustancia Propia / Queratocitos de la Córnea / Fibroblastos Idioma: En Revista: Bull Exp Biol Med Año: 2021 Tipo del documento: Article