Palm Oil-Rich Diet Affects Murine Liver Proteome and S-Palmitoylome.
Int J Mol Sci
; 22(23)2021 Dec 03.
Article
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| MEDLINE
| ID: mdl-34884899
ABSTRACT
Palmitic acid (C160) is the most abundant saturated fatty acid in animals serving as a substrate in synthesis and ß-oxidation of other lipids, and in the modification of proteins called palmitoylation. The influence of dietary palmitic acid on protein S-palmitoylation remains largely unknown. In this study we performed high-throughput proteomic analyses of a membrane-enriched fraction of murine liver to examine the influence of a palm oil-rich diet (HPD) on S-palmitoylation of proteins. HPD feeding for 4 weeks led to an accumulation of C160 and C181 fatty acids in livers which disappeared after 12-week feeding, in contrast to an accumulation of C160 in peritoneal macrophages. Parallel proteomic studies revealed that HPD feeding induced a sequence of changes of the level and/or S-palmitoylation of diverse liver proteins involved in fatty acid, cholesterol and amino acid metabolism, hemostasis, and neutrophil degranulation. The HPD diet did not lead to liver damage, however, it caused progressing obesity, hypercholesterolemia and hyperglycemia. We conclude that the relatively mild negative impact of such diet on liver functioning can be attributed to a lower bioavailability of palm oil-derived C160 vs. that of C181 and the efficiency of mechanisms preventing liver injury, possibly including dynamic protein S-palmitoylation.
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MEDLINE
Asunto principal:
Aceite de Soja
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Aceite de Palma
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Ácido Palmítico
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Proteómica
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Idioma:
En
Revista:
Int J Mol Sci
Año:
2021
Tipo del documento:
Article