Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW.
Proc Natl Acad Sci U S A
; 119(10): e2118227119, 2022 03 08.
Article
en En
| MEDLINE
| ID: mdl-35238645
ABSTRACT
SignificanceHost-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes and quantitative proteomics to identify the target of epinephrine and the α-adrenoceptor agonist phenylephrine in live cells of the aquatic pathogen Vibrio campbellii. Consequently, we have discovered the coupling protein CheW, which is in the center of the chemotaxis signaling network, as a target of both molecules. We not only demonstrate direct ligand binding to CheW but also elucidate how this affects chemotactic control. These findings are pivotal for further research on hormone-specific effects on bacterial behavior.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Vibrio
/
Catecolaminas
/
Factores Quimiotácticos
/
Quimiotaxis
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2022
Tipo del documento:
Article