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Tig1 regulates proximo-distal identity during salamander limb regeneration.
Oliveira, Catarina R; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G; Gates, Phillip B; Walters, Hannah E; Petzold, Andreas; Arce, Hernan; Cordoba, Rodrigo C; Subramanian, Elaiyaraja; Chara, Osvaldo; Tanaka, Elly M; Simon, András; Yun, Maximina H.
Afiliación
  • Oliveira CR; Technische Universität Dresden, CRTD/Center for Regenerative Therapies Dresden, Dresden, Germany.
  • Knapp D; Technische Universität Dresden, CRTD/Center for Regenerative Therapies Dresden, Dresden, Germany. dunja.knapp@tu-dresden.de.
  • Elewa A; Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
  • Gerber T; European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • Gonzalez Malagon SG; Institute of Structural and Molecular Biology, University College London, London, UK.
  • Gates PB; Biomedical Research Institute, Foundation for Research and Technology, University of Ioannina Campus, 45115, Ioannina, Greece.
  • Walters HE; Institute of Structural and Molecular Biology, University College London, London, UK.
  • Petzold A; Technische Universität Dresden, CRTD/Center for Regenerative Therapies Dresden, Dresden, Germany.
  • Arce H; Technische Universität Dresden, CRTD/Center for Regenerative Therapies Dresden, Dresden, Germany.
  • Cordoba RC; Systems Biology Group, Institute of Physics of Liquids and Biological Systems, National Scientific and Technical Research Council (CONICET) and University of La Plata, La Plata, Argentina.
  • Subramanian E; Instituto de Tecnología, Universidad Argentina de la Empresa (UADE), Buenos Aires, Argentina.
  • Chara O; Systems Biology Group, Institute of Physics of Liquids and Biological Systems, National Scientific and Technical Research Council (CONICET) and University of La Plata, La Plata, Argentina.
  • Tanaka EM; Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
  • Simon A; Systems Biology Group, Institute of Physics of Liquids and Biological Systems, National Scientific and Technical Research Council (CONICET) and University of La Plata, La Plata, Argentina.
  • Yun MH; Instituto de Tecnología, Universidad Argentina de la Empresa (UADE), Buenos Aires, Argentina.
Nat Commun ; 13(1): 1141, 2022 03 03.
Article en En | MEDLINE | ID: mdl-35241664
Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed positional identity. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting Hoxa13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Urodelos / Extremidades Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2022 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Urodelos / Extremidades Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2022 Tipo del documento: Article