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Serum proteomic analysis reveals the cardioprotective effects of Shexiang Baoxin Pill and Suxiao Jiuxin Pill in a rat model of acute myocardial infarction.
Song, Nixue; Lu, Dayun; Wu, Gaosong; Wang, Shisheng; Zeng, Yuanyuan; Zhao, Jing; Meng, Qian; He, Han; Chen, Linlin; Zhu, Hongwen; Liu, Aijun; Li, Houkai; Shen, Xiaoxu; Zhang, Weidong; Zhou, Hu.
Afiliación
  • Song N; CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Lu D; CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Wu G; Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
  • Wang S; Frontiers Science Center for Disease-related Molecular Network, Institutes for Systems Genetics, Key Lab of Transplant Engineering and Immunology, MOH, West China Hospital, Sichuan University, Chengdu, 610041, China.
  • Zeng Y; Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100700, China.
  • Zhao J; Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100700, China.
  • Meng Q; CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
  • He H; CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
  • Chen L; Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
  • Zhu H; CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
  • Liu A; School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
  • Li H; Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
  • Shen X; Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100700, China. Electronic address: jhxiaoxushen@163.com.
  • Zhang W; Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; School of Pharmacy, Second Military Medical University, Shanghai, 200433, China. Electronic address: wdzhangy@hotmail.com.
  • Zhou H; CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: zhouhu@simm.ac.cn.
J Ethnopharmacol ; 293: 115279, 2022 Jul 15.
Article en En | MEDLINE | ID: mdl-35405256
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Shexiang Baoxin Pill (SBP) and Suxiao Jiuxin Pill (SJP) are traditional Chinese medicines used to treat cardiovascular disease (CVD) in China. However, the mechanism of their therapeutic effect on CVD has not been clearly elucidated yet.

AIMS:

The aim of this study is to investigate the cardioprotective effect of SBP and SJP in the treatment of acute myocardial infarction (AMI) model rats by applying serum proteomic approach. MATERIALS AND

METHODS:

The rat model of AMI was generated by ligating the left anterior descending coronary artery. 42 rats were randomly divided into four groups sham-operating (Sham, n = 10) group, model (Mod, n = 8) group, Shexiang Baoxin pills pretreatment (SBP, n = 12) group and Suxiao Jiuxin pills pretreatment (SJP, n = 12) group. Data Independent Acquisition (DIA) proteomic approach was utilized to investigate the serum proteome from the rat individuals. The differentially expressed proteins were subsequently obtained with bioinformatic analysis.

RESULTS:

DIA-MS identified 415 proteins within 42 samples, and 84 differentially expressed proteins may contribute to the therapeutic effects of SBP and SJP. GOBP and KEGG pathway analysis of 84 differentially expressed proteins revealed that the proteins were mainly involved in platelet activation and adhesion processes. All 84 differentially expressed proteins presented the same changing tendency in the SBP and SJP groups when compared with the Mod group. Among these 84 proteins, 25 proteins were found to be related to CVD. Among these 25 proteins, ACTB, ACTG1, FGA, FGB, FGG, PF4 and VWF were found to be involved in platelet aggregation and activation. FN1, HSPA5 and YWHAZ were associated with adhesion.

CONCLUSIONS:

The results of our study suggest that the cardioprotective effects of SBP and SJP are achieved through the modulation of focal adhesion, platelet activation pathways.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Medicamentos Herbarios Chinos / Infarto del Miocardio Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Ethnopharmacol Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Medicamentos Herbarios Chinos / Infarto del Miocardio Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Ethnopharmacol Año: 2022 Tipo del documento: Article