Antimicrobial peptide WAM-1: a promising antibacterial and anti-inflammatory drug against carbapenem-resistant Klebsiella pneumoniae.
J Antimicrob Chemother
; 77(7): 1903-1911, 2022 06 29.
Article
en En
| MEDLINE
| ID: mdl-35474013
ABSTRACT
BACKGROUND:
The emergence and spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) pose a threat to public health. Antimicrobial peptides provide a new treatment option for CRKP infections.OBJECTIVES:
We studied antibacterial activities of WAM-1 against CRKP in vitro and in vivo and explored its possible mechanism. We verified safety and factors affecting antibacterial effect. Furthermore, anti-inflammatory effects were investigated.METHODS:
We selected eight CRKP and eight carbapenem-susceptible K. pneumoniae to explore the antibacterial activity of WAM-1 by broth microdilution (BMD). The possible mechanism was investigated by alkaline phosphatase leakage and propidium iodide (PI). We evaluated safety of WAM-1 by cytotoxicity and haemolysis and effects of temperature and serum on the antibacterial activity. We investigated in vivo efficacy of WAM-1 by the Galleria mellonella infection model. We investigated the effect of WAM-1 on TNF-α.RESULTS:
BMD showed that WAM-1 had a good antibacterial effect with MICs of 2-4â mg/L and MBCs of 4-8â mg/L. RT-qPCR showed that WAM-1 could inhibit the expression of TNF-α. The cytotoxicity and haemolysis test proved that WAM-1 had certain potential application in vivo. Alkaline phosphatase leakage and PI fluorescence showed that WAM-1 was highly likely to exert an antibacterial effect by destroying bacterial membrane. The G. mellonella infection model suggested that WAM-1 may have a good therapeutic effect in vivo. Temperature had little effect on the activity of WAM-1. Serum, however, reduced WAM-1 activity.CONCLUSIONS:
WAM-1 has good antibacterial effect and potential anti-inflammatory effect on infection caused by CRKP.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Infecciones por Klebsiella
/
Enterobacteriaceae Resistentes a los Carbapenémicos
/
Péptidos Antimicrobianos
/
Antiinflamatorios
/
Antibacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Antimicrob Chemother
Año:
2022
Tipo del documento:
Article