Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodonticinduced external apical root resorption in a rat model.
Mol Med Rep
; 26(1)2022 Jul.
Article
en En
| MEDLINE
| ID: mdl-35593309
ABSTRACT
The aim of the present study was to investigate the alterations in the formation of cementocytes in response to orthodontic forces and to evaluate the contribution of these cells in the biological changes of tooth movement and associated root resorption. A total of 90 Sprague Dawley rats were randomly assigned to the control, high force, and low force groups. Intrusion forces of 10 and 50 g were applied on the rat molar to induce tooth intrusion. The tooth movement was observed from 0 to 14 days by microcomputed tomography, bone histometric analysis, tartrateresistant acid phosphatase staining, as well as reverse transcriptionquantitative PCR and immunofluorescence staining assays. The results suggested that under low force conditions, osteoclasts were distributed at a higher frequency on the bone side than on the root side. Under high force conditions, both sides suffered osteoclast infiltration. In the low force group, the cementocytes exhibited downregulated sclerostin (SOST) and osteoprotegerin (OPG) mRNA levels and a lower receptor activator of nuclear factorκB ligand (RANKL)/OPG ratio over a certain period of time. The expression levels of these genes were lower compared with those of the osteocytes at each timepoint. In the high force group, both cementocytes and osteocytes upregulated the SOST and RANKL/OPG ratio on days 7 and 14, while the cementocytes expressed higher levels of SOST mRNA than those noted in the osteocytes. These data suggested that cementocytes responded to the orthodontic force via modulation of the RANKL/OPG ratio and SOST expression. The biological response of cementocytes contributed to the mechanotransduction and homoeostasis of the roots under compression. Excessive forces may act as a negative factor of this regulatory role. These results expand our knowledge on the function of cementocytes.
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Base de datos:
MEDLINE
Asunto principal:
Resorción Radicular
/
Osteoprotegerina
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Idioma:
En
Revista:
Mol Med Rep
Año:
2022
Tipo del documento:
Article