Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance.
Chem Commun (Camb)
; 58(51): 7184-7187, 2022 Jun 23.
Article
en En
| MEDLINE
| ID: mdl-35670542
ABSTRACT
Small molecules in solution may interfere with mechanistic investigations, as they can affect the stability of catalytic states and produce off-cycle states that can be mistaken for catalytically relevant species. Here we show that the hydride state (Hhyd), a proposed central intermediate in the catalytic cycle of [FeFe]-hydrogenase, can be formed in wild-type [FeFe]-hydrogenases treated with H2 in absence of other, non-biological, reductants. Moreover, we reveal a new state with unclear role in catalysis induced by common low pH buffers.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Hidrogenasas
/
Proteínas Hierro-Azufre
Idioma:
En
Revista:
Chem Commun (Camb)
Asunto de la revista:
QUIMICA
Año:
2022
Tipo del documento:
Article