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Analysis of the Biological Properties of Blood Plasma Protein with GcMAF Functional Activity.
Dolgova, Evgeniya V; Kirikovich, Svetlana S; Levites, Evgeniy V; Ruzanova, Vera S; Proskurina, Anastasia S; Ritter, Genrikh S; Taranov, Oleg S; Varaksin, Nikolay A; Ryabicheva, Tatiana G; Leplina, Olga Yu; Ostanin, Alexandr A; Chernykh, Elena R; Bogachev, Sergey S.
Afiliación
  • Dolgova EV; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Kirikovich SS; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Levites EV; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Ruzanova VS; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Proskurina AS; Department of Natural Sciences, Novosibirsk National Research State University, 630090 Novosibirsk, Russia.
  • Ritter GS; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Taranov OS; Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Varaksin NA; State Research Center of Virology and Biotechnology "Vector", 630559 Koltsovo, Russia.
  • Ryabicheva TG; JSC "Vector-Best", 630559 Koltsovo, Russia.
  • Leplina OY; JSC "Vector-Best", 630559 Koltsovo, Russia.
  • Ostanin AA; Research Institute of Fundamental and Clinical Immunology, 630099 Novosibirsk, Russia.
  • Chernykh ER; Research Institute of Fundamental and Clinical Immunology, 630099 Novosibirsk, Russia.
  • Bogachev SS; Research Institute of Fundamental and Clinical Immunology, 630099 Novosibirsk, Russia.
Int J Mol Sci ; 23(15)2022 Jul 22.
Article en En | MEDLINE | ID: mdl-35897653
ABSTRACT
The main problem related to the studies focusing on group-specific component protein-derived macrophage-activating factor (GcMAF) is the lack of clarity about changes occurring in different types of macrophages and related changes in their properties under the effect of GcMAF in various clinical conditions. We analyzed the antitumor therapeutic properties of GcMAF in a Lewis carcinoma model in two clinical conditions untreated tumor lesion and tumor resorption after exposure to Karanahan therapy. GcMAF is formed during site-specific deglycosylation of vitamin D3 binding protein (DBP). DBP was obtained from the blood of healthy donors using affinity chromatography on a column with covalently bound actin. GcMAF-related factor (GcMAF-RF) was converted in a mixture with induced lymphocytes through the cellular enzymatic pathway. The obtained GcMAF-RF activates murine peritoneal macrophages (p < 0.05), induces functional properties of dendritic cells (p < 0.05) and promotes in vitro polarization of human M0 macrophages to M1 macrophages (p < 0.01). Treatment of whole blood cells with GcMAF-RF results in active production of both pro- and anti-inflammatory cytokines. It is shown that macrophage activation by GcMAF-RF is inhibited by tumor-secreted factors. In order to identify the specific antitumor effect of GcMAF-RF-activated macrophages, an approach to primary reduction of humoral suppressor activity of the tumor using the Karanahan therapy followed by macrophage activation in the tumor-associated stroma (TAS) was proposed. A prominent additive effect of GcMAF-RF, which enhances the primary immune response activation by the Karanahan therapy, was shown in the model of murine Lewis carcinoma. Inhibition of the suppressive effect of TAS is the main condition required for the manifestation of the antitumor effect of GcMAF-RF. When properly applied in combination with any chemotherapy, significantly reducing the humoral immune response at the advanced tumor site, GcMAF-RF is a promising antitumor therapeutic agent that additively destroys the pro-tumor properties of macrophages of the tumor stroma.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Proteína de Unión a Vitamina D / Carcinoma / Factores Activadores de Macrófagos Tipo de estudio: Prognostic_studies Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Proteína de Unión a Vitamina D / Carcinoma / Factores Activadores de Macrófagos Tipo de estudio: Prognostic_studies Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article