Danggui Niantong granules ameliorate rheumatoid arthritis by regulating intestinal flora and promoting mitochondrial apoptosis.
Pharm Biol
; 60(1): 1606-1615, 2022 Dec.
Article
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| MEDLINE
| ID: mdl-35961296
ABSTRACT
CONTEXT Danggui Niantong Granules (DGNTG) are a valid and reliable traditional herbal formula, commonly used in clinical practice to treat rheumatoid arthritis (RA). However, the mechanism of its effect on RA remains unclear. OBJECTIVE:
An investigation of the therapeutic effects of DGNTG on RA. MATERIALS ANDMETHODS:
Twenty-four male Sprague-Dawley (SD) rats were divided into four groups control, model, DGNTG (2.16 g/kg, gavage), methotrexate (MTX) (1.35 mg/kg, gavage) for 28 days. The morphology of synovial and ankle tissues was observed by haematoxylin-eosin staining. The responses of mitochondrial apoptosis were assessed by qPCR, Western blotting and immunohistochemical staining. Rat faeces were analysed by 16S rRNA sequencing.RESULTS:
Our results showed that DGNTG treatment reduced AI scores (7.83 ± 0.37 vs. 4.67 ± 0.47, p < 0.01) and paw volumes (7.63 ± 0.17 vs. 6.13 ± 0.11, p < 0.01) compared with the model group. DGNTG also increased the expression of Bax (0.34 ± 0.03 vs. 0.73 ± 0.03, p < 0.01), cytochrome c (CYTC) (0.24 ± 0.02 vs. 0.64 ± 0.01, p < 0.01) and cleaved caspase-9 (0.24 ± 0.04 vs. 0.83 ± 0.08, p < 0.01), and decreased bcl-2 (1.70 ± 0.11 vs. 0.60 ± 0.09, p < 0.01) expression. DGNTG treatment regulated the structure of gut microbiota. DISCUSSION ANDCONCLUSIONS:
DGNTG ameliorated RA by promoting mitochondrial apoptosis, which may be associated with regulating gut microbiota structure. DGNTG can be used as a supplement and alternative drug for the treatment of RA; its ability to prevent RA deserves further study.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Artritis Experimental
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Artritis Reumatoide
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Medicamentos Herbarios Chinos
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Apoptosis
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Microbioma Gastrointestinal
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Pharm Biol
Año:
2022
Tipo del documento:
Article