Your browser doesn't support javascript.
loading
Heterogeneity of the NIH3T3 Fibroblast Cell Line.
Rahimi, Amir Mohammad; Cai, Mingfang; Hoyer-Fender, Sigrid.
Afiliación
  • Rahimi AM; Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology-Developmental Biology, Göttingen Center for Molecular Biosciences (GZMB), Ernst-Caspari-Haus, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
  • Cai M; Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology-Developmental Biology, Göttingen Center for Molecular Biosciences (GZMB), Ernst-Caspari-Haus, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
  • Hoyer-Fender S; Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology-Developmental Biology, Göttingen Center for Molecular Biosciences (GZMB), Ernst-Caspari-Haus, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
Cells ; 11(17)2022 08 28.
Article en En | MEDLINE | ID: mdl-36078083
The embryonic mouse fibroblast cell line NIH3T3 is widely used in life science research, including the study of cell cycle control and primary cilia. Fibroblasts are the most important cell type in connective tissue, as they produce components of the extracellular matrix and determine tissue architecture. However, they are very heterogeneous and consist of subtypes specific to their organ of residence, among others. The NIH3T3 cell line was derived from whole mouse embryos that developed to pre-birth and is therefore most likely composed of different fibroblast subtypes. Furthermore, prolonged proliferation may have influenced their cellular composition. A heterogeneous cell population is unsuitable for any sophisticated research project. We found that the proportion of ciliated cells in the total NIH3T3 cell population was highly variable and asked whether this was a consequence of cellular heterogeneity and what molecular signatures were associated with it. We have established sub-cell lines by clonal expansion of single cells and characterized them morphologically and molecularly. Eventually, a myofibroblast-like and a fibroblast-like cell line were generated that differ in ciliation and proliferation. These homogeneous cell lines are valuable for a more detailed study of their molecular signatures, not least to uncover further the molecular pathways that contribute to the formation of the primary cilium.
Asunto(s)
Palabras clave

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Miofibroblastos / Fibroblastos Idioma: En Revista: Cells Año: 2022 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Miofibroblastos / Fibroblastos Idioma: En Revista: Cells Año: 2022 Tipo del documento: Article