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Mimicry of embryonic circulation enhances the hoxa hemogenic niche and human blood development.
Li, Jingjing; Lao, Osmond; Bruveris, Freya F; Wang, Liyuan; Chaudry, Kajal; Yang, Ziqi; Farbehi, Nona; Ng, Elizabeth S; Stanley, Edouard G; Harvey, Richard P; Elefanty, Andrew G; Nordon, Robert E.
Afiliación
  • Li J; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia.
  • Lao O; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia.
  • Bruveris FF; Murdoch Children's Research Institute, The Royal Children's Hospital, Flemington Road, Parkville, VIC 3052, Australia; Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC 3052, Australia; The Novo Nordisk Foundation Center for Stem C
  • Wang L; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia.
  • Chaudry K; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia.
  • Yang Z; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia.
  • Farbehi N; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia; Victor Chang Cardiac Research Institute. School of Clinical Medicine, University of New South Wales, Sydney, NSW, Australia; Garvan Weizmann Centre for Cellular Genomics, Garvan Institute of Medical Res
  • Ng ES; Murdoch Children's Research Institute, The Royal Children's Hospital, Flemington Road, Parkville, VIC 3052, Australia; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
  • Stanley EG; Murdoch Children's Research Institute, The Royal Children's Hospital, Flemington Road, Parkville, VIC 3052, Australia; Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC 3052, Australia; The Novo Nordisk Foundation Center for Stem C
  • Harvey RP; Victor Chang Cardiac Research Institute. School of Clinical Medicine, University of New South Wales, Sydney, NSW, Australia.
  • Elefanty AG; Murdoch Children's Research Institute, The Royal Children's Hospital, Flemington Road, Parkville, VIC 3052, Australia; Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC 3052, Australia; The Novo Nordisk Foundation Center for Stem C
  • Nordon RE; Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia. Electronic address: r.nordon@unsw.edu.au.
Cell Rep ; 40(11): 111339, 2022 09 13.
Article en En | MEDLINE | ID: mdl-36103836
ABSTRACT
Precursors of the adult hematopoietic system arise from the aorta-gonad-mesonephros (AGM) region shortly after the embryonic circulation is established. Here, we develop a microfluidic culture system to mimic the primitive embryonic circulation and address the hypothesis that circulatory flow and shear stress enhance embryonic blood development. Embryonic (HOXA+) hematopoiesis was derived from human pluripotent stem cells and induced from mesoderm by small-molecule manipulation of TGF-ß and WNT signaling (SB/CHIR). Microfluidic and orbital culture promoted the formation of proliferative CD34+RUNX1C-GFP+SOX17-mCHERRY+ precursor cells that were released into the artificial circulation from SOX17+ arterial-like structures. Single-cell transcriptomic analysis delineated extra-embryonic (yolk sac) and HOXA+ embryonic blood differentiation pathways. SB/CHIR and circulatory flow enhance hematopoiesis by the formation of proliferative HOXA+RUNX1C+CD34+ precursor cells that differentiate into monocyte/macrophage, granulocyte, erythrocyte, and megakaryocyte progenitors.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Hematopoyesis / Mesonefro Idioma: En Revista: Cell Rep Año: 2022 Tipo del documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Hematopoyesis / Mesonefro Idioma: En Revista: Cell Rep Año: 2022 Tipo del documento: Article