CD44-targeted nanoparticles with GSH-responsive activity as powerful therapeutic agents against breast cancer.
Int J Biol Macromol
; 221: 1491-1503, 2022 Nov 30.
Article
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| MEDLINE
| ID: mdl-36130642
ABSTRACT
DOX-loaded nanoparticles able to actively target CD44-receptors and respond to redox stimuli were proposed as non-conventional chemotherapeutic strategy in breast cancer. A covalent conjugate of human serum albumin and hyaluronic acid was prepared and assembled by a GSH-mediated desolvation in disulfide-crosslinked solid nanoparticles with mean diameter of 120 nm ± 3.4. The effective internalization of nanoparticles in cancer cells via CD44-receptors, together with the more efficient intracellular release, resulted in a significant increase of drug efficacy, with IC50 reduced from 0.9959 and 2.516 µg mL-1 to 0.4014 and 0.3094 µg mL-1 for MCF-7 and MDA-MB-231, respectively. Conversely, no enhancement in drug toxicity was recorded in healthy MCF-10A cells. The efficacy of the proposed formulation was further investigated in the different biological steps involved in metastasis process, paving the way for further in vivo experiments.
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MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Nanopartículas
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2022
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Article