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Targeted Mutagenesis of the Multicopy Myrosinase Gene Family in Allotetraploid Brassica juncea Reduces Pungency in Fresh Leaves across Environments.
Karlson, Dale; Mojica, Julius P; Poorten, Thomas J; Lawit, Shai J; Jali, Sathya; Chauhan, Raj Deepika; Pham, Gina M; Marri, Pradeep; Guffy, Sharon L; Fear, Justin M; Ochsenfeld, Cherie A; Lincoln Chapman, Tracey A; Casamali, Bruno; Venegas, Jorge P; Kim, Hae Jin; Call, Ashley; Sublett, William L; Mathew, Lolita G; Shariff, Aabid; Watts, Joseph M; Mann, Mike; Hummel, Aaron; Rapp, Ryan.
Afiliación
  • Karlson D; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Mojica JP; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Poorten TJ; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Lawit SJ; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Jali S; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Chauhan RD; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Pham GM; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Marri P; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Guffy SL; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Fear JM; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Ochsenfeld CA; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Lincoln Chapman TA; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Casamali B; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Venegas JP; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Kim HJ; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Call A; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Sublett WL; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Mathew LG; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Shariff A; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Watts JM; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Mann M; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Hummel A; Pairwise, 807 E Main St., Durham, NC 27701, USA.
  • Rapp R; Pairwise, 807 E Main St., Durham, NC 27701, USA.
Plants (Basel) ; 11(19)2022 Sep 23.
Article en En | MEDLINE | ID: mdl-36235360
ABSTRACT
Recent breeding efforts in Brassica have focused on the development of new oilseed feedstock crop for biofuels (e.g., ethanol, biodiesel, bio-jet fuel), bio-industrial uses (e.g., bio-plastics, lubricants), specialty fatty acids (e.g., erucic acid), and producing low glucosinolates levels for oilseed and feed meal production for animal consumption. We identified a novel opportunity to enhance the availability of nutritious, fresh leafy greens for human consumption. Here, we demonstrated the efficacy of disarming the 'mustard bomb' reaction in reducing pungency upon the mastication of fresh tissue-a major source of unpleasant flavor and/or odor in leafy Brassica. Using gene-specific mutagenesis via CRISPR-Cas12a, we created knockouts of all functional copies of the type-I myrosinase multigene family in tetraploid Brassica juncea. Our greenhouse and field trials demonstrate, via sensory and biochemical analyses, a stable reduction in pungency in edited plants across multiple environments. Collectively, these efforts provide a compelling path toward boosting the human consumption of nutrient-dense, fresh, leafy green vegetables.
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Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Plants (Basel) Año: 2022 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Plants (Basel) Año: 2022 Tipo del documento: Article