Simple production of resilin-like protein hydrogels using the Brevibacillus secretory expression system and column-free purification.

Homma, Toshimasa; Terui, Shu; Yokoyama, Fuki; Okino, Saki; Ohta, Sora; Kato, Chihiro; Haraguchi, Naoki; Fujisawa, Ikuhide; Itsuno, Shinichi; Ang, Lily Zuin Ping

Homma, Toshimasa; Terui, Shu; Yokoyama, Fuki; Okino, Saki; Ohta, Sora; Kato, Chihiro; Haraguchi, Naoki; Fujisawa, Ikuhide; Itsuno, Shinichi; Ang, Lily Zuin Ping.

Afiliación

Homma T; Division of Chemical Engineering and Biotechnology, National Institute of Technology, Ichinoseki College, Ichinoseki Iwate, Japan.
Terui S; Division of Chemical Engineering and Biotechnology, National Institute of Technology, Ichinoseki College, Ichinoseki Iwate, Japan.
Yokoyama F; Division of Chemical Engineering and Biotechnology, National Institute of Technology, Ichinoseki College, Ichinoseki Iwate, Japan.
Okino S; Division of Chemical Engineering and Biotechnology, National Institute of Technology, Ichinoseki College, Ichinoseki Iwate, Japan.
Ohta S; Division of Chemical Engineering and Biotechnology, National Institute of Technology, Ichinoseki College, Ichinoseki Iwate, Japan.
Kato C; Division of Chemical Engineering and Biotechnology, National Institute of Technology, Ichinoseki College, Ichinoseki Iwate, Japan.
Haraguchi N; Department of Applied Chemistry & Life Science, Toyohashi University of Technology, Toyohashi, Aichi, Japan.
Fujisawa I; Department of Applied Chemistry & Life Science, Toyohashi University of Technology, Toyohashi, Aichi, Japan.
Itsuno S; Department of Applied Chemistry & Life Science, Toyohashi University of Technology, Toyohashi, Aichi, Japan.
Ang LZP; National Institute of Technology, Gifu College, Motosu, Gifu, Japan.

Biotechnol Bioeng ; 120(1): 194-202, 2023 01.

Article en En | MEDLINE | ID: mdl-36253915

Asunto(s)

Brevibacillus; Animales; Brevibacillus/genética; Brevibacillus/metabolismo; Drosophila melanogaster/metabolismo; Hidrogeles; Proteínas de Insectos/genética; Proteínas Recombinantes; Medios de Cultivo/metabolismo

Palabras clave

Brevibacillus choshinensis; column-free purification; extracellular expression; photocrosslinking; resilin-like proteins; three-dimensional hydrogel

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Brevibacillus Idioma: En Revista: Biotechnol Bioeng Año: 2023 Tipo del documento: Article

Texto completo

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PubMed Links

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Brevibacillus Idioma: En Revista: Biotechnol Bioeng Año: 2023 Tipo del documento: Article