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Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells.
Xu, Xiao; Yin, Fengyue; Guo, Mengyu; Gan, Guohong; Lin, Guanzhong; Wen, Chengwen; Wang, Junsheng; Song, Pengbo; Wang, Jinling; Qi, Zhong-Quan; Zhong, Chuan-Qi.
Afiliación
  • Xu X; Department of Biomedical Engineering, Medical College of Guangxi University, Nanning, Guangxi, China.
  • Yin F; Department of Biomedical Engineering, Medical College of Guangxi University, Nanning, Guangxi, China.
  • Guo M; Department of Emergency, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China.
  • Gan G; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
  • Lin G; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
  • Wen C; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
  • Wang J; Department of Emergency, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China.
  • Song P; Department of Biomedical Engineering, Medical College of Guangxi University, Nanning, Guangxi, China.
  • Wang J; Department of emergency, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
  • Qi ZQ; Department of Biomedical Engineering, Medical College of Guangxi University, Nanning, Guangxi, China.
  • Zhong CQ; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Proteomics ; 23(1): e2200204, 2023 01.
Article en En | MEDLINE | ID: mdl-36408942
ABSTRACT
Exosomes derived from mesenchymal stem cells (MSCs) have been used for cancer treatment, however, an in-depth analysis of the exosomal proteomes is lacking. In this manuscript, we use the diaPASEF (parallel accumulation serial fragmentation combined with the data-independent acquisition) method to quantify exosomes derived from human umbilical cord mesenchymal stem cells (UCMSCs) and rat bone marrow stem cells (BMSCs), resulting in identification of 4200 human proteins and 5362 rat proteins. Comparison of human exosomal proteins and total cellular proteins reveals that some proteins exist in the exosomes exclusively that can be served as potential markers for exosomes. Quantitative proteomic analysis of exosomes from different passages of BMSCs shows that the proteins involved in TGF-ß signaling pathway are regulated in abundance, which could be markers for the therapeutic ability of BMSC exosomes. Collectively, the data presented by this study can be a resource for further study of exosome research.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: MicroARNs / Exosomas / Células Madre Mesenquimatosas Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: MicroARNs / Exosomas / Células Madre Mesenquimatosas Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article