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Generation of induced pluripotent stem cells from three individuals with Huntington's disease.
Miller, Duncan C; Lisowski, Pawel; Lickfett, Selene; Mlody, Barbara; Bünning, Miriam; Genehr, Carolin; Ulrich, Claas; Wanker, Erich E; Diecke, Sebastian; Priller, Josef; Prigione, Alessandro.
Afiliación
  • Miller DC; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany.
  • Lisowski P; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Magdalenka n/Warsaw, Poland; Neurops
  • Lickfett S; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
  • Mlody B; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
  • Bünning M; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
  • Genehr C; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
  • Ulrich C; Department of Dermatology and Allergy, Charité - Universitätsmedizin Berlin, Germany.
  • Wanker EE; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
  • Diecke S; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany. Electronic address: Sebastian.diecke@mdc-berlin.de.
  • Priller J; Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité - Universitätsmedizin Berlin, Germany; DZNE, Berlin, Germany; University of Edinburgh and UK DRI, Edinburgh, UK; Department of Psychiatry and Psychotherapy, School of Medicine, Technical University Munich, Germany. Electronic address: j
  • Prigione A; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany. Electronic address: alessandro.prigione@hhu.de.
Stem Cell Res ; 65: 102976, 2022 12.
Article en En | MEDLINE | ID: mdl-36434993
ABSTRACT
Huntington's disease (HD) is a neurodegenerative disorder caused by abnormal glutamine (Q) expansion in the huntingtin protein due to elongated CAG repeats in the gene HTT. We used non-integrative episomal plasmids to generate induced pluripotent stem cells (iPSCs) from three individuals affected by HD CH1 (58Q), and two twin brothers CH3 (44Q) and CH4 (44Q). The iPSC lines exhibited one healthy HTT allele and one with elongated CAG repeats, as confirmed by PCR and sequencing. All iPSC lines expressed pluripotency markers, exhibited a normal karyotype, and generated cells of the three germ layers in vitro.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Enfermedad de Huntington / Células Madre Pluripotentes Inducidas / Proteína Huntingtina Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article
Texto completo
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XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Enfermedad de Huntington / Células Madre Pluripotentes Inducidas / Proteína Huntingtina Idioma: En Revista: Stem Cell Res Año: 2022 Tipo del documento: Article