Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.

Venken, Koen J T; Matinyan, Nick; Gonzalez, Yezabel; Dierick, Herman A

Venken, Koen J T; Matinyan, Nick; Gonzalez, Yezabel; Dierick, Herman A.

Afiliación

Venken KJT; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Matinyan N; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas.
Gonzalez Y; Integrative Molecular Biomedical Sciences Graduate Program, Baylor College of Medicine, Houston, Texas.
Dierick HA; McNair Medical Institute at The Robert and Janice McNair Foundation, Baylor College of Medicine, Houston, Texas.

Curr Protoc ; 3(2): e675, 2023 Feb.

Article en En | MEDLINE | ID: mdl-36757632

Asunto(s)

Drosophila melanogaster; Técnicas de Transferencia de Gen; Animales; Drosophila melanogaster/genética; Animales Modificados Genéticamente; ADN; Drosophila/genética; Genómica; Clonación Molecular; Células Clonales

Palabras clave

Drosophila melanogaster; P[acman]; bacterial artificial chromosome; gene expression analysis; selection; serial recombineering cloning; transgenesis

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Técnicas de Transferencia de Gen / Drosophila melanogaster Idioma: En Revista: Curr Protoc Año: 2023 Tipo del documento: Article

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