Solution structure of the DNA binding domain of Arabidopsis transcription factor WRKY11.
Biochem Biophys Res Commun
; 653: 133-139, 2023 04 23.
Article
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| MEDLINE
| ID: mdl-36868077
ABSTRACT
The Arabidopsis WRKY11 (AtWRKY11) protein is an important transcription factor involved in plant response to biotic and abiotic stresses. Its DNA-binding domain specifically binds to gene promoter regions harboring the W-box consensus motif. Herein we report the high-resolution structure of the AtWRKY11 DNA-binding domain (DBD) determined by solution NMR spectroscopy. The results show that AtWRKY11-DBD adopts an all-ß fold comprising five ß-strands packed in an antiparallel topology, stabilized by a zinc-finger motif. Structural comparison reveals that the long ß1-ß2 loop shows the highest structural variation from other available WRKY domain structures. Moreover, this loop was further found to contribute to the binding between AtWRKY11-DBD and W-box DNA. Our current study provides atomic-level structural basis for further understanding the structure-function relationship of plant WRKY proteins.
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MEDLINE
Asunto principal:
Arabidopsis
/
Proteínas de Arabidopsis
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2023
Tipo del documento:
Article