A 3D culture system improves the yield of MSCs-derived extracellular vesicles and enhances their therapeutic efficacy for heart repair.
Biomed Pharmacother
; 161: 114557, 2023 May.
Article
en En
| MEDLINE
| ID: mdl-36963364
ABSTRACT
BACKGROUND:
Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs), due to their inner functional substances, have shown great value in treating acute myocardial infarction (AMI). However, their clinical application is limited by a low yield. In the present study, we cultured EVs using a hollow fiber bioreactor-based three-dimensional (3D) system, and assessed their therapeutic effectiveness on AMI.METHODS:
The MSCs separated from fresh human umbilical cord were planted into the flasks of two systems two-dimensional (2D) culture and hollow-fiber-bioreactor based 3D culture. EVs were extracted from the culture supernatants. Characteristics and yields of EVs from two culture systems, namely 2D-EVs and 3D-EVs, were compared. A rat model of AMI was built up to assess their therapeutic efficacy on AMI.RESULTS:
The yield of 3D-EVs was higher, with biofunctions similar to those of 2D-EVs. 3D-EVs repressed the apoptosis of cardiomyocytes, facilitated angiogenesis, and regulated the transition of macrophage subpopulations after myocardial infarction, and eventually improved cardiac function in the AMI rats.CONCLUSIONS:
The hollow fiber 3D culture system can increase the yield of MSCs-derived EVs to render a strong cardioprotective effect in AMI rats.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Madre Mesenquimatosas
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Vesículas Extracelulares
/
Infarto del Miocardio
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biomed Pharmacother
Año:
2023
Tipo del documento:
Article