Adenovirus core protein V reinforces the capsid and enhances genome release from disrupted particles.
Sci Adv
; 9(14): eade9910, 2023 04 07.
Article
en En
| MEDLINE
| ID: mdl-37027464
ABSTRACT
Out of the three core proteins in human adenovirus, protein V is believed to connect the inner capsid surface to the outer genome layer. Here, we explored mechanical properties and in vitro disassembly of particles lacking protein V (Ad5-ΔV). Ad5-ΔV particles were softer and less brittle than the wild-type ones (Ad5-wt), but they were more prone to release pentons under mechanical fatigue. In Ad5-ΔV, core components did not readily diffuse out of partially disrupted capsids, and the core appeared more condensed than in Ad5-wt. These observations suggest that instead of condensing the genome, protein V antagonizes the condensing action of the other core proteins. Protein V provides mechanical reinforcement and facilitates genome release by keeping DNA connected to capsid fragments that detach during disruption. This scenario is in line with the location of protein V in the virion and its role in Ad5 cell entry.
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Base de datos:
MEDLINE
Asunto principal:
Adenovirus Humanos
/
Cápside
Idioma:
En
Revista:
Sci Adv
Año:
2023
Tipo del documento:
Article