Your browser doesn't support javascript.
loading
Melatonin inhibits senescence-associated melanin pigmentation through the p53-TYR pathway in human primary melanocytes and the skin of C57BL/6 J mice after UVB irradiation.
Ma, Li-Ping; Liu, Meng-Meng; Liu, Fang; Sun, Bo; Wang, Si-Nian; Chen, Jie; Yu, Hui-Jie; Yan, Juan; Tian, Mei; Gao, Ling; Liu, Qing-Jie.
Afiliación
  • Ma LP; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China.
  • Liu MM; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China.
  • Liu F; Department of Dermatology, Chaoyang Hospital, Capital Medical University, 100020, Beijing, China.
  • Sun B; PLA Rocket Force Characteristic Medical Center, 100088, Beijing, China.
  • Wang SN; PLA Rocket Force Characteristic Medical Center, 100088, Beijing, China.
  • Chen J; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China.
  • Yu HJ; PLA Rocket Force Characteristic Medical Center, 100088, Beijing, China.
  • Yan J; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China.
  • Tian M; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China.
  • Gao L; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China. gaoling@nirp.chinacdc.cn.
  • Liu QJ; China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, 100088, Beijing, China. liuqingjie@nirp.chinacdc.cn.
J Mol Med (Berl) ; 101(5): 581-593, 2023 05.
Article en En | MEDLINE | ID: mdl-37032347
ABSTRACT
UVB exposure accelerates skin aging and pigmentation. Melatonin effectively regulates tyrosinase (TYR) activity and aging. The purpose of this study was to determine the association between premature senescence and pigmentation, and the mechanism of melanin synthesis effected by melatonin. Primary melanocytes were extracted and identified from the male foreskin. To inhibit TYR expression, primary melanocytes were transduced with the lentivirus pLKD-CMV-EGFP-2A-Puro-U6-TYR. The wild-type TYR(+/+) and TYR(-/-) or TYR(+/-) knockout C57BL/6 J mice were used to determine the role of TYR on melanin synthesis in vivo. Results showed that UVB-induced melanin synthesis is dependent on TYR in primary melanocytes and mice. Furthermore, in primary melanocytes pretreated with Nutlin-3 or PFT-α to up or downregulate p53, results showed that premature senescence and melanin synthesis increased in primary melanocytes after UVB irradiation at 80 mJ/cm2, and further increased after being treated with Nutlin-3, while significantly decreased with PFT-α. In addition, melatonin inhibited UVB-induced premature senescence associated with inactivation of p53 and phosphorylation of p53 on Ser15 (ser-15), a decrease of melanin synthesis accompanied by reduced TYR expression. Moreover, skin erythema and pigmentation induced by UVB were reduced in the dorsal and ear skin of mice topically pretreated with 2.5% melatonin. These indicate that melatonin inhibits UVB-induced senescence-associated pigmentation via the p53-TYR pathway in primary melanocytes and prevents pigmentation obviously in the dorsal and ear skin of C57BL/6 J mice after UVB irradiation. KEY MESSAGES P53 links UVB irradiation-induced senescence and senescence-associated pigmentation and regulates TYR in primary melanocytes after UVB irradiation. Melatonin inhibits senescence-associated pigmentation through the p53-TYR pathway in primary melanocytes. Melatonin prevents skin erythema and melanin pigmentation induced by UVB irradiation in the dorsal and ear skin of C57BL/6J mice.
Asunto(s)
Palabras clave

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Melaninas / Melatonina Tipo de estudio: Risk_factors_studies Idioma: En Revista: J Mol Med (Berl) Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2023 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Melaninas / Melatonina Tipo de estudio: Risk_factors_studies Idioma: En Revista: J Mol Med (Berl) Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2023 Tipo del documento: Article