Convection and extracellular matrix binding control interstitial transport of extracellular vesicles.
J Extracell Vesicles
; 12(4): e12323, 2023 04.
Article
en En
| MEDLINE
| ID: mdl-37073802
Extracellular vesicles (EVs) influence a host of normal and pathophysiological processes in vivo. Compared to soluble mediators, EVs can traffic a wide range of proteins on their surface including extracellular matrix (ECM) binding proteins, and their large size (â¼30-150 nm) limits diffusion. We isolated EVs from the MCF10 series-a model human cell line of breast cancer progression-and demonstrated increasing presence of laminin-binding integrins α3ß1 and α6ß1 on the EVs as the malignant potential of the MCF10 cells increased. Transport of the EVs within a microfluidic device under controlled physiological interstitial flow (0.15-0.75 µm/s) demonstrated that convection was the dominant mechanism of transport. Binding of the EVs to the ECM enhanced the spatial concentration and gradient, which was mitigated by blocking integrins α3ß1 and α6ß1. Our studies demonstrate that convection and ECM binding are the dominant mechanisms controlling EV interstitial transport and should be leveraged in nanotherapeutic design.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Laminina
/
Vesículas Extracelulares
Idioma:
En
Revista:
J Extracell Vesicles
Año:
2023
Tipo del documento:
Article