Chromosomal instability and its effect on cell lines.
Cancer Rep (Hoboken)
; 6(6): e1822, 2023 06.
Article
en En
| MEDLINE
| ID: mdl-37095005
ABSTRACT
BACKGROUND:
Cancer cell lines are invaluable model systems for biomedical research because they provide an almost unlimited supply of biological materials. However, there is considerable skepticism regarding the reproducibility of data derived from these in vitro models. RECENTFINDINGS:
Chromosomal instability (CIN) is one of the primary issues associated with cell lines, which can cause genetic heterogeneity and unstable cell properties within a cell population. Many of these problems can be avoided with some precautions. Here we review the underlying causes of CIN, including merotelic attachment, telomere dysfunction, DNA damage response defects, mitotic checkpoint defects and cell cycle disturbances.CONCLUSION:
In this review we summarize studies highlighting the consequences of CIN in various cell lines and provide suggestions on monitoring and controlling CIN during cell culture.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Mitosis
/
Neoplasias
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Cancer Rep (Hoboken)
Año:
2023
Tipo del documento:
Article