Evaluation of the Sysmex UF-4000i urine analyzer as a screening test to rule out urinary tract infection and reduce urine cultures. / Évaluation de l'analyseur Sysmex UF-4000i comme test de dépistage pour exclusion des infections urinaires et diminution des mises en culture
Ann Biol Clin (Paris)
; 81(2): 156-161, 2023 05 16.
Article
en En, Fr
| MEDLINE
| ID: mdl-37144792
ABSTRACT
INTRODUCTION:
Urinary tract infection (UTI) diagnosis by urine culture is time- and labor- consuming. In the Ibn Rochd microbiology laboratory, up to 70% of urine culture samples yield no growth or insignificant growth.OBJECTIVE:
To evaluate the new generation of Sysmex UF-4000i fluorescence flow cytometry analyzer with a blue semiconducting laser as a method to rule out negative urine samples for UTI, in comparison of urine culture. MATERIAL ANDMETHODS:
Flow cytometry and microbiological analysis were performed on 502 urine samples included in the study. We used ROC analysis to determine cutoff points at which optimal sensitivity and specificity are achieved for clinical use.RESULTS:
Our results showed that bacteria count at a cut-off of 100/µL, and/or the leucocytes count ≥ 45/µL are the optimal indicator for positive culture results. At these cut off, bacteria sensitivity (SE), specificity (SP), Positive predictive value (PPV) and negative predictive value (NPV) were 97,3%, 95%, 87,8% and 98,8% respectively. For leucocytes, SE, SP, PPV and NPV were 99,1%, 95,8%, 88,6% and 99,7% respectively. DISCUSSION ANDCONCLUSION:
The bacterial and leucocytes counts generated by UF-4000i analysis may be useful in our context as a rapid screening to exclude UTI by reducing about 70% of urines cultures and then workload. Nevertheless, further validation is needed for different patient groups especially with urological disease or immunocompromised patients.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Infecciones Urinarias
/
Urinálisis
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
/
Fr
Revista:
Ann Biol Clin (Paris)
Año:
2023
Tipo del documento:
Article