Your browser doesn't support javascript.
loading
Characterization of elements, PAHs, AhR-activity and pro-inflammatory responses of road tunnel-derived particulate matter in human hepatocyte-like and bronchial epithelial cells.
Holme, Jørn A; Låg, Marit; Skuland, Tonje; Parenicová, Martina; Ciganek, Miroslav; Penciková, Katerina; Grytting, Vegard Sæter; Neca, Jiri; Øvrevik, Johan; Mariussen, Espen; Jørgensen, Rikke Bramming; Refsnes, Magne; Machala, Miroslav.
Afiliación
  • Holme JA; Department of Air quality and Noise, Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway.
  • Låg M; Department of Air quality and Noise, Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway. Electronic address: marit.lag@fhi.no.
  • Skuland T; Department of Air quality and Noise, Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway.
  • Parenicová M; Department of Pharmacology and Toxicology, Veterinary Research Institute, 62100 Brno, Czech Republic.
  • Ciganek M; Department of Pharmacology and Toxicology, Veterinary Research Institute, 62100 Brno, Czech Republic.
  • Penciková K; Department of Pharmacology and Toxicology, Veterinary Research Institute, 62100 Brno, Czech Republic.
  • Grytting VS; Department of Air quality and Noise, Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway.
  • Neca J; Department of Pharmacology and Toxicology, Veterinary Research Institute, 62100 Brno, Czech Republic.
  • Øvrevik J; Department of Biosciences, Faculty of Mathematics and Natural Sciences, University of Oslo, PO Box 1066 Blindern, 0316 Oslo, Norway; Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway.
  • Mariussen E; Department of Air quality and Noise, Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway.
  • Jørgensen RB; Department of Industrial Economics and Technology Management, Norwegian University of Science and Technology, NTNU, Trondheim, Norway.
  • Refsnes M; Department of Air quality and Noise, Division of Climate and Environmental Health, Norwegian Institute of Public Health, PO Box 222 Skøyen, 0213 Oslo, Norway.
  • Machala M; Department of Pharmacology and Toxicology, Veterinary Research Institute, 62100 Brno, Czech Republic.
Toxicol In Vitro ; 90: 105611, 2023 Aug.
Article en En | MEDLINE | ID: mdl-37164185
The aims were to characterize the content of elements and polycyclic aromatic hydrocarbons (PAHs) in size-separated particulate matter (PM) sampled in a road tunnel, estimate the contribution of PAHs to the toxic potential, and measure the pro-inflammatory potential of PM samples and extracts with increasing polarity. Several elements/metals previously associated with cytokine responses were found. Based on PAHs levels and published PAHs potency, the calculated mutagenic and carcinogenic activities of size-separated samples were somewhat lower for coarse than fine and ultrafine PM. The AhR-activity of the corresponding PM extracts measured in an AhR-luciferase reporter model (human hepatocytes) were more similar. The highest AhR-activity was found in the neutral (parent and alkylated PAHs) and polar (oxy-PAHs) fractions, while the semi-polar fractions (mono-nitrated-PAHs) had only weak activity. The neutral and polar aromatic fractions from coarse and fine PM were also found to induce higher pro-inflammatory responses and CYP1A1 expression in human bronchial epithelial cells (HBEC3-KT) than the semi-polar fractions. Fine PM induced higher pro-inflammatory responses than coarse PM. AhR-inhibition reduced cytokine responses induced by parent PM and extracts of both size fractions. Contributors to the toxic potentials include PAHs and oxy-PAHs, but substantial contributions from other organic compounds and/or metals are likely.
Asunto(s)
Palabras clave

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes Atmosféricos Idioma: En Revista: Toxicol In Vitro Asunto de la revista: TOXICOLOGIA Año: 2023 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes Atmosféricos Idioma: En Revista: Toxicol In Vitro Asunto de la revista: TOXICOLOGIA Año: 2023 Tipo del documento: Article