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Identification of optimal reference genes in golden Syrian hamster with ethanol- and palmitoleic acid-induced acute pancreatitis using quantitative real-time polymerase chain reaction.
Miao, Jinxin; Kang, Le; Lan, Tianfeng; Wang, Jianyao; Wu, Siqing; Jia, Yifan; Xue, Xia; Guo, Haoran; Wang, Pengju; Li, Yan.
Afiliación
  • Miao J; Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou, China.
  • Kang L; Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou, China.
  • Lan T; Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China.
  • Wang J; Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China.
  • Wu S; Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou, China.
  • Jia Y; Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou, China.
  • Xue X; Henan Key Laboratory of Helicobacter pylori and Microbiota and Gastrointestinal Cancer, Marshall Medical Research Center, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Guo H; Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China.
  • Wang P; Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China.
  • Li Y; Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou, China.
Animal Model Exp Med ; 6(6): 609-618, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37202901
BACKGROUND: Acute pancreatitis (AP) is a severe disorder that leads to high morbidity and mortality. Appropriate reference genes are important for gene analysis in AP. This study sought to study the expression stability of several reference genes in the golden Syrian hamster, a model of AP. METHODS: AP was induced in golden Syrian hamster by intraperitoneal injection of ethanol (1.35 g/kg) and palmitoleic acid (2 mg/kg). The expression of candidate genes, including Actb, Gapdh, Eef2, Ywhaz, Rps18, Hprt1, Tubb, Rpl13a, Nono, and B2m, in hamster pancreas at different time points (1, 3, 6, 9, and 24 h) posttreatment was analyzed using quantitative polymerase chain reaction. The expression stability of these genes was calculated using BestKeeper, Comprehensive Delta CT, NormFinder, and geNorm algorithms and RefFinder software. RESULTS: Our results show that the expression of these reference genes fluctuated during AP, of which Ywhaz and Gapdh were the most stable genes, whereas Tubb, Eef2, and Actb were the least stable genes. Furthermore, these genes were used to normalize the expression of TNF-α messenger ribonucleic acid in inflamed pancreas. CONCLUSIONS: In conclusion, Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in Syrian hamster.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Pancreatitis Tipo de estudio: Diagnostic_studies Idioma: En Revista: Animal Model Exp Med Año: 2023 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Pancreatitis Tipo de estudio: Diagnostic_studies Idioma: En Revista: Animal Model Exp Med Año: 2023 Tipo del documento: Article