Nanobody-based immunomagnetic separation platform for rapid isolation and detection of Salmonella enteritidis in food samples.
Food Chem
; 424: 136416, 2023 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-37247600
ABSTRACT
Rapid separation and identification of Salmonella enteritidis (S. enteritidis) in food is of great importance to prevent outbreaks of foodborne diseases. Herein, by using O and H antigens as targets, an epitope-based bio-panning strategy was applied to isolate specific nanobodies towards S. enteritidis. This method constitutes an efficient way to obtain specific antibody fragments and test pairwise nanobodies. On this basis, a double nanobody-based sandwich enzyme-linked immunosorbent assay (ELISA) coupled with immunomagnetic separation (IMS) was developed to rapid enrich and detect S. enteritidis in food. The detection limit of the IMS-ELISA was 3.2 × 103 CFU/mL. In addition, 1 CFU of S. enteritidis in food samples can be detected after 4-h cultivation, which was shortened by 2 h after IMS. The IMS-ELISA strategy could avoid matrix interference and shorten the enrichment culture time, which has great potential for application in monitoring bacterial contamination in food.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Separación Inmunomagnética
/
Anticuerpos de Dominio Único
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Food Chem
Año:
2023
Tipo del documento:
Article