Exploring alternative pathways for the in vitro establishment of the HOPAC cycle for synthetic CO2 fixation.
Sci Adv
; 9(24): eadh4299, 2023 06 16.
Article
en En
| MEDLINE
| ID: mdl-37315145
ABSTRACT
Nature has evolved eight different pathways for the capture and conversion of CO2, including the Calvin-Benson-Bassham cycle of photosynthesis. Yet, these pathways underlie constrains and only represent a fraction of the thousands of theoretically possible solutions. To overcome the limitations of natural evolution, we introduce the HydrOxyPropionyl-CoA/Acrylyl-CoA (HOPAC) cycle, a new-to-nature CO2-fixation pathway that was designed through metabolic retrosynthesis around the reductive carboxylation of acrylyl-CoA, a highly efficient principle of CO2 fixation. We realized the HOPAC cycle in a step-wise fashion and used rational engineering approaches and machine learning-guided workflows to further optimize its output by more than one order of magnitude. Version 4.0 of the HOPAC cycle encompasses 11 enzymes from six different organisms, converting ~3.0 mM CO2 into glycolate within 2 hours. Our work moves the hypothetical HOPAC cycle from a theoretical design into an established in vitro system that forms the basis for different potential applications.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Dióxido de Carbono
/
Procedimientos de Cirugía Plástica
Idioma:
En
Revista:
Sci Adv
Año:
2023
Tipo del documento:
Article