Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections.
Cell Rep
; 42(7): 112766, 2023 07 25.
Article
en En
| MEDLINE
| ID: mdl-37421618
ABSTRACT
Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations is challenging. To overcome this, we rationally select the highly conserved peptides from the consensus amino acid sequence of the globular head domains of neuraminidase. Inspired by the B cell receptor evolution process, a reliable sequential immunization regimen is designed to result in immuno-focusing by steering bulk immune responses to a selected region where broadly protective B lymphocyte epitopes reside. After priming neuraminidase protein-specific antibody responses in C57BL/6 or BALB/c inbred mice strains by immunization or pre-infection, boost immunizations with certain neuraminidase-derived peptide-keyhole limpet hemocyanin conjugates significantly strengthened serum neuraminidase inhibition activities and cross-protections. Overall, this study provides proof of concept for a peptide-based sequential immunization strategy for achieving targeted induction of cross-protective antibody response, which provides references for designing universal vaccines against other highly variable pathogens.
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Base de datos:
MEDLINE
Asunto principal:
Vacunas contra la Influenza
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Infecciones por Orthomyxoviridae
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Gripe Humana
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Subtipo H1N1 del Virus de la Influenza A
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Subtipo H5N1 del Virus de la Influenza A
Idioma:
En
Revista:
Cell Rep
Año:
2023
Tipo del documento:
Article