Murine Models of Salmonella Infection.
Curr Protoc
; 3(7): e824, 2023 Jul.
Article
en En
| MEDLINE
| ID: mdl-37478288
ABSTRACT
The pathogen Salmonella enterica encompasses a range of bacterial serovars that cause intestinal inflammation and systemic infections in humans. Mice are a widely used infection model due to their relative simplicity and versatility. Here, we provide standardized protocols for culturing the prolific zoonotic pathogen S. enterica serovar Typhimurium for intragastric inoculation of mice to model colitis or systemic dissemination, along with techniques for direct extraintestinal infection. Furthermore, we present procedures for quantifying pathogen burden and for characterizing the immune response by analyzing tissue pathology, inflammatory markers, and immune cells from intestinal tissues. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 Murine colitis model utilizing oral streptomycin pretreatment and oral S. Typhimurium administration Basic Protocol 2 Intraperitoneal injection of S. Typhimurium for modeling extraintestinal infection Support Protocol 1 Preparation of S. Typhimurium inoculum Support Protocol 2 Preparation of mixed S. Typhimurium inoculum for competitive infection Basic Protocol 3 Assessment of S. Typhimurium burden Support Protocol 3 Preservation and pathological assessment of S. Typhimurium-infected tissues Support Protocol 4 Measurement of inflammatory marker expression in intestinal tissues by qPCR Support Protocol 5 Preparation of intestinal content for inflammatory marker quantification by ELISA Support Protocol 6 Immune cell isolation from Salmonella-infected intestinal tissues.
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Base de datos:
MEDLINE
Asunto principal:
Infecciones por Salmonella
/
Colitis
Idioma:
En
Revista:
Curr Protoc
Año:
2023
Tipo del documento:
Article