Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons.
STAR Protoc
; 4(3): 102486, 2023 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-37515763
Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks. For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).1.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Madre Pluripotentes Inducidas
Tipo de estudio:
Guideline
/
Prognostic_studies
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article