The administration of rhBmal1 reduces sleep deprivation-induced anxiety and cognitive impairment in mice.
World J Biol Psychiatry
; 25(1): 43-53, 2024.
Article
en En
| MEDLINE
| ID: mdl-37640026
BACKGROUND: In mammals, circadian rhythms control metabolism, immunological response and reproductive processes. Bmal1 (brain and muscle Arnt-like protein-1) is a key element in the regulation of circadian rhythms. METHODS: This investigation explores the pathophysiological effects of sleep deprivation in a mouse model as well as the potential underlying mechanisms. A mouse sleep deprivation model was constructed using a modified multi-platform water environment method. The anxiety-like behaviours of mice were assessed by the open field test and elevated plus maze, and the cognitive function of mice was tested by the nest-building test. The expression levels of targeted genes were determined by Western blotting assay and RT-qPCR assay. RESULTS: We found that sleep deprivation profoundly enhanced anxiety levels and impaired cognitive function in mice. Sleep deprivation also reduced the expression levels of Bmal1 and BDNF (brain-derived neurotrophic factor) and increased oxidative stress in the hippocampus of mice. The intraperitoneal injection of human recombinant rhBmal1 protein alleviated sleep deprivation-induced anxiety and cognitive impairment, restored Bmal1 and BDNF levels, and reduced oxidative stress in the hippocampus of mice. CONCLUSIONS: rhBmal1 treatment might serve as a potential therapy for mitigating sleep deprivation-related unfavourable symptoms.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Privación de Sueño
/
Disfunción Cognitiva
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
World J Biol Psychiatry
Asunto de la revista:
PSIQUIATRIA
Año:
2024
Tipo del documento:
Article