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Analysing biomarkers in oral fluid from pigs: influence of collection strategy and age of the pig.
Ornelas, Mario Andre S; López-Martínez, María José; Franco-Martínez, Lorena; Cerón, José J; Ortín-Bustillo, Alba; Rubio, Camila Peres; Manzanilla, Edgar Garcia.
Afiliación
  • Ornelas MAS; Pig Development Department, Teagasc Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland. andreorne@gmail.com.
  • López-Martínez MJ; School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland. andreorne@gmail.com.
  • Franco-Martínez L; Interdisciplinary Laboratory of Clinical Analysis, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia (Interlab-UMU), University of Murcia, Campus de Espinardo s/n, Murcia, 30100, Spain.
  • Cerón JJ; Pig Development Department, Teagasc Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland.
  • Ortín-Bustillo A; Interdisciplinary Laboratory of Clinical Analysis, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia (Interlab-UMU), University of Murcia, Campus de Espinardo s/n, Murcia, 30100, Spain.
  • Rubio CP; Interdisciplinary Laboratory of Clinical Analysis, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia (Interlab-UMU), University of Murcia, Campus de Espinardo s/n, Murcia, 30100, Spain.
  • Manzanilla EG; Interdisciplinary Laboratory of Clinical Analysis, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia (Interlab-UMU), University of Murcia, Campus de Espinardo s/n, Murcia, 30100, Spain.
Porcine Health Manag ; 9(1): 39, 2023 Aug 30.
Article en En | MEDLINE | ID: mdl-37649074
ABSTRACT
BACKGROUND AND

OBJECTIVES:

Oral fluid (OF) is an easy-to-collect, inexpensive, fast and non-invasive sample to characterize health and welfare status of the pig. However, further standardisation of the collection methods is needed in order to use it regularly in veterinary practice. Cotton ropes are routinely used to collect OF for pathogen detection but they may not be optimal for biomarker analysis due to sample contamination. This study compared two methods (cotton ropes and sponges) to collect porcine OF for biomarker analysis. A panel of 11 biomarkers of stress, inflammation, sepsisimmunity, redox status and general homeostasis was studied. MATERIALS AND

METHODS:

Eighteen farrow-to-finish pig farms were included in the study. In each farm, three (for sponges) or four pens of pigs (for ropes) were sampled at four age categories the week after weaning (5 weeks), before (11-12 weeks) and after (12-13 weeks) moving to finisher facility and the week before slaughter (22-25 weeks). In total, 288 OF samples were collected with cotton ropes and 216 with sponges and analysed for the biomarkers cortisol, alpha-amylase, oxytocin (stress), haptoglobin (inflammation), procalcitonin (sepsis), adenosine deaminase, immunoglobulin G (immune system), ferric reducing antioxidant power (redox status), and creatine kinase, lactate dehydrogenase and total protein (general homeostasis). Samples were also scored visually for dirtiness using a score from 1 (clean) to 5 (very dirty).

RESULTS:

Rope-collected OF had higher levels of dirtiness (3.7 ± 0.04) compared to sponge-collected OF (2.7 ± 0.15) and had higher values than sponges for cortisol, procalcitonin, oxytocin, haptoglobin, total protein, lactate dehydrogenase and ferric reducing antioxidant power. All biomarkers decreased in value with age. Immunoglobulin G did not perform well for any of the two collection methods. DISCUSSION AND

CONCLUSION:

The results showed a clear effect of age on the biomarkers in OF collected with both, sponges or ropes. Sponges provided a cleaner sample than cotton ropes for biomarker analysis. Both methods are easy to apply under the commercial conditions in pig farms although sponges may take more time in early weaner stages. From a practical point of view, sampling with sponges achieved the best combination of reduced sampling time and low contamination.
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Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Porcine Health Manag Año: 2023 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Porcine Health Manag Año: 2023 Tipo del documento: Article