Your browser doesn't support javascript.
loading
Development of a Method for Isolation of Mature Cardiomyocytes from Human Heart Biopsy Specimens.
Kovalenko, S G; Frolova, Sh R; Kramkova, V K; Berezovskii, A K; Popov, M A; Shumakov, D V; Zybin, D I; Agafonov, E G; Dontsov, V V; Agladze, K I.
Afiliación
  • Kovalenko SG; Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
  • Frolova SR; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
  • Kramkova VK; Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
  • Berezovskii AK; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
  • Popov MA; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
  • Shumakov DV; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
  • Zybin DI; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
  • Agafonov EG; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
  • Dontsov VV; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
  • Agladze KI; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
Bull Exp Biol Med ; 175(4): 585-591, 2023 Aug.
Article en En | MEDLINE | ID: mdl-37768452
ABSTRACT
To increase the yield of living cells and their survival, studies were carried out to optimize the method for isolating cardiomyocytes from biopsy specimens excised from the right atrial appendages. It was found that creatine, blebbistatin, and taurine are necessary components of the buffer solution during cardiomyocyte isolation, and that composition of the solutions is a more important factor than their oxygenation.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Taurina / Miocitos Cardíacos Idioma: En Revista: Bull Exp Biol Med Año: 2023 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Taurina / Miocitos Cardíacos Idioma: En Revista: Bull Exp Biol Med Año: 2023 Tipo del documento: Article