A robust NIR fluorescence-activated probe for peroxynitrite imaging in cells and mice osteoarthritis models.
Anal Biochem
; 682: 115338, 2023 12 01.
Article
en En
| MEDLINE
| ID: mdl-37802174
ABSTRACT
Osteoarthritis (OA) is the most common type of joint disease, which is difficult to treat, but early standardized diagnosis and treatment can effectively alleviate the pain and symptoms of patients. Therefore, it is important to construct an effective tool to assist in the early diagnosis and evaluation of the therapeutic effect of OA. In this work, a near-infrared (NIR) fluorescence-activated fluorescent probe, YB-1, was constructed for the evaluation of the diagnostic and therapeutic efficacy of OA via detection and imaging of the biomarker of ONOO- in inflammatory cells and mice osteoarthritis models. YB-1 exhibited high selectivity, high sensitivity, and a high ratio yield (I668/I0) fluorescence increasing (â¼30 folds). Besides, YB-1 can be used effectively to image endogenous and exogenous ONOO- in living human chondrocytes cells (TC28a2), as well as to evaluate the effect of drug (Chrysosplenol D, CD) treatment in IL-1ß-induced inflammatory cells model. Interestingly, YB-1 was available for OONO- imaging analysis in the collagenase-induced mice OA models and assessment of the effect of CD treatment in mice OA models, with good results. Thus, the newly constructed YB-1 is a powerful molecular tool for the diagnosis and treatment of OA-related diseases.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Osteoartritis
/
Colorantes Fluorescentes
Tipo de estudio:
Screening_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2023
Tipo del documento:
Article