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Fatigue-resistant hydrogel optical fibers enable peripheral nerve optogenetics during locomotion.
Liu, Xinyue; Rao, Siyuan; Chen, Weixuan; Felix, Kayla; Ni, Jiahua; Sahasrabudhe, Atharva; Lin, Shaoting; Wang, Qianbin; Liu, Yuanyuan; He, Zhigang; Xu, Jingyi; Huang, Sizhe; Hong, Eunji; Yau, Todd; Anikeeva, Polina; Zhao, Xuanhe.
Afiliación
  • Liu X; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. xyliu@msu.edu.
  • Rao S; Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI, USA. xyliu@msu.edu.
  • Chen W; Department of Biomedical Engineering, University of Massachusetts Amherst, Amherst, MA, USA. syrao@binghamton.edu.
  • Felix K; Department of Biomedical Engineering, Binghamton University, State University of New York, Binghamton, NY, USA. syrao@binghamton.edu.
  • Ni J; Department of Biomedical Engineering, University of Massachusetts Amherst, Amherst, MA, USA.
  • Sahasrabudhe A; Department of Biomedical Engineering, University of Massachusetts Amherst, Amherst, MA, USA.
  • Lin S; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Wang Q; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Liu Y; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • He Z; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Xu J; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Huang S; Department of Mechanical Engineering, Michigan State University, East Lansing, MI, USA.
  • Hong E; Department of Biomedical Engineering, University of Massachusetts Amherst, Amherst, MA, USA.
  • Yau T; Department of Biomedical Engineering, Binghamton University, State University of New York, Binghamton, NY, USA.
  • Anikeeva P; Somatosensation and Pain Unit, National Institute of Dental and Craniofacial Research, National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD, USA.
  • Zhao X; F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
Nat Methods ; 20(11): 1802-1809, 2023 Nov.
Article en En | MEDLINE | ID: mdl-37857906
ABSTRACT
We develop soft and stretchable fatigue-resistant hydrogel optical fibers that enable optogenetic modulation of peripheral nerves in naturally behaving animals during persistent locomotion. The formation of polymeric nanocrystalline domains within the hydrogels yields fibers with low optical losses of 1.07 dB cm-1, Young's modulus of 1.6 MPa, stretchability of 200% and fatigue strength of 1.4 MPa against 30,000 stretch cycles. The hydrogel fibers permitted light delivery to the sciatic nerve, optogenetically activating hindlimb muscles in Thy1ChR2 mice during 6-week voluntary wheel running assays while experiencing repeated deformation. The fibers additionally enabled optical inhibition of pain hypersensitivity in an inflammatory model in TRPV1NpHR mice over an 8-week period. Our hydrogel fibers offer a motion-adaptable and robust solution to peripheral nerve optogenetics, facilitating the investigation of somatosensation.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Fibras Ópticas / Optogenética Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2023 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Fibras Ópticas / Optogenética Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2023 Tipo del documento: Article