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Imaging cellular forces with photonic crystals.
Li, Qiwei; Chen, Zaozao; Zhang, Ying; Ding, Shuang; Ding, Haibo; Wang, Luping; Xie, Zhuoying; Fu, Yifu; Wei, Mengxiao; Liu, Shengnan; Chen, Jialun; Wang, Xuan; Gu, Zhongze.
Afiliación
  • Li Q; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Chen Z; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Zhang Y; Institute of Biomaterials and Medical Devices, Southeast University, 215163, Suzhou, Jiangsu, China.
  • Ding S; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Ding H; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Wang L; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Xie Z; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Fu Y; Faculty of Sports Science, Ningbo University, 315211, Ningbo, China.
  • Wei M; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Liu S; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Chen J; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Wang X; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
  • Gu Z; State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
Nat Commun ; 14(1): 7369, 2023 Nov 14.
Article en En | MEDLINE | ID: mdl-37963911
ABSTRACT
Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vertical cell forces over a wide field of view (1.3 mm ⨯ 1.0 mm, a 10 ⨯ objective image) at high speed (about 20 frames per second) without references. The photonic crystal hydrogel substrate (PCS) converts micro-nano deformations into perceivable color changes, enabling in situ visualization and quantification of tiny vertical cell forces with high throughput. It enabled long-term, cross-scale monitoring from subcellular focal adhesions to tissue-level cell sheets and aggregates.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Fotones / Adhesiones Focales Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Fotones / Adhesiones Focales Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article