Protocol for screening α-synuclein PET tracer candidates in vitro and ex vivo.
STAR Protoc
; 5(1): 102788, 2024 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-38117656
ABSTRACT
Alpha-synuclein (α-Syn) positron emission tomography (PET) imaging is a valuable approach for diagnosing and monitoring synucleinopathies-related diseases, such as Parkinson disease. Here, we present a protocol for screening potential α-Syn PET tracers using in vitro and ex vivo approaches. We describe steps for employing recombinant pre-formed fibrils and conducting screening procedures on neuronal models, mouse models, and patients' brain tissue sections to assess the specificity and selectivity of the candidate compounds. For complete details on the use and execution of this protocol, please refer to Xiang et al. (2023).1.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Enfermedad de Parkinson
/
Alfa-Sinucleína
Idioma:
En
Revista:
STAR Protoc
/
STAR protocols
Año:
2024
Tipo del documento:
Article