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Exosomes from Von Hippel-Lindau-Null Cancer Cells Promote Metastasis in Renal Cell Carcinoma.
Flora, Kailey; Ishihara, Moe; Zhang, Zhicheng; Bowen, Elizabeth S; Wu, Aimee; Ayoub, Tala; Huang, Julian; Cano-Ruiz, Celine; Jackson, Maia; Reghu, Kaveeya; Ayoub, Yasmeen; Zhu, Yazhen; Tseng, Hsian-Rong; Zhou, Z Hong; Hu, Junhui; Wu, Lily.
Afiliación
  • Flora K; Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
  • Ishihara M; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
  • Zhang Z; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
  • Bowen ES; Department of Computational and Systems Biology, University of California, Los Angeles, CA 90095, USA.
  • Wu A; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
  • Ayoub T; Department of Physiology, University of California, Los Angeles, CA 90095, USA.
  • Huang J; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
  • Cano-Ruiz C; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
  • Jackson M; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
  • Reghu K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
  • Ayoub Y; School of Medicine, Saint Louis University, St. Louis, MO 63104, USA.
  • Zhu Y; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
  • Tseng HR; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
  • Zhou ZH; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
  • Hu J; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
  • Wu L; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Int J Mol Sci ; 24(24)2023 Dec 09.
Article en En | MEDLINE | ID: mdl-38139136
ABSTRACT
Exosomes are extracellular vesicles that modulate essential physiological and pathological signals. Communication between cancer cells that express the von Hippel-Lindau (VHL) tumor suppressor gene and those that do not is instrumental to distant metastasis in renal cell carcinoma (RCC). In a novel metastasis model, VHL(-) cancer cells are the metastatic driver, while VHL(+) cells receive metastatic signals from VHL(-) cells and undergo aggressive transformation. This study investigates whether exosomes could be mediating metastatic crosstalk. Exosomes isolated from paired VHL(+) and VHL(-) cancer cell lines were assessed for physical, biochemical, and biological characteristics. Compared to the VHL(+) cells, VHL(-) cells produce significantly more exosomes that augment epithelial-to-mesenchymal transition (EMT) and migration of VHL(+) cells. Using a Cre-loxP exosome reporter system, the fluorescent color conversion and migration were correlated with dose-dependent delivery of VHL(-) exosomes. VHL(-) exosomes even induced a complete cascade of distant metastasis when added to VHL(+) tumor xenografts in a duck chorioallantoic membrane (dCAM) model, while VHL(+) exosomes did not. Therefore, this study supports that exosomes from VHL(-) cells could mediate critical cell-to-cell crosstalk to promote metastasis in RCC.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Carcinoma de Células Renales / Exosomas / Neoplasias Renales Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Carcinoma de Células Renales / Exosomas / Neoplasias Renales Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article