Randomly barcoded transposon mutant libraries for gut commensals II: Applying libraries for functional genetics.
Cell Rep
; 43(1): 113519, 2024 01 23.
Article
en En
| MEDLINE
| ID: mdl-38142398
ABSTRACT
The critical role of the intestinal microbiota in human health and disease is well recognized. Nevertheless, there are still large gaps in our understanding of the functions and mechanisms encoded in the genomes of most members of the gut microbiota. Genome-scale libraries of transposon mutants are a powerful tool to help us address this gap. Recent advances in barcoded transposon mutagenesis have dramatically lowered the cost of mutant fitness determination in hundreds of in vitro and in vivo experimental conditions. In an accompanying review, we discuss recent advances and caveats for the construction of pooled and arrayed barcoded transposon mutant libraries in human gut commensals. In this review, we discuss how these libraries can be used across a wide range of applications, the technical aspects involved, and expectations for such screens.
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Base de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
Idioma:
En
Revista:
Cell Rep
Año:
2024
Tipo del documento:
Article