LncRNA H19 Participates in Leukemia Inhibitory Factor Mediated Stemness Promotion in Colorectal Cancer Cells.

Zhu, Min; Yu, Ruihong; Liu, Yirui; Geng, Xiaoqing; Liu, Qiong; Liu, Shuaitong; Zhu, Yunhe; Li, Gang; Guo, Yang; Xi, Xueyan; Du, Boyu

Zhu, Min; Yu, Ruihong; Liu, Yirui; Geng, Xiaoqing; Liu, Qiong; Liu, Shuaitong; Zhu, Yunhe; Li, Gang; Guo, Yang; Xi, Xueyan; Du, Boyu.

Afiliación

Zhu M; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Yu R; Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Liu Y; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Geng X; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Liu Q; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Liu S; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Zhu Y; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Li G; Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Guo Y; Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Xi X; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China.
Du B; Institute of Basic Medical Sciences, Hubei University of Medicine, No.30 Renmin Nanlu, Shiyan, 442000, Hubei, China. xixueyan2001@126.com.

Biochem Genet ; 2024 Jan 10.

Article en En | MEDLINE | ID: mdl-38198021

ABSTRACT

ABSTRACT

Colorectal cancer (CRC) is a common human malignancy and the third leading cause of cancer-related death worldwide. Cancer stem cells (CSCs) were considered to play important roles in the genesis and development of many tumors. In recent years, it has been observed that leukemia inhibitory factor (LIF) might be involved in the regulation of stemness in cancer cells. In this study, we observed that LIF could increase the spheroid formation and stemness marker expression (inculding Nanog and SOX2) in CRC cell lines, such as HCT116 and Caco2 cells. Meanwhile, we also observed that LIF could upregulate LncRNA H19 expression via PI3K/AKT pathway. Knockdown of the expression of LncRNA H19 could decrease the spheroid formation and SOX2 expression in LIF-treated HCT116 and Caco2 cells, and thereby LncRNA H19 knockdown could compensate for the stemness enhancement effects induced by LIF. Our results indicated that LncRNA H19 might participate in the stemness promotion of LIF in CRC cells.

Palabras clave

AKT; Cancer stem cells; Colorectal cancer; H19; Leukemia inhibitory factor

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