Quantum dots-based "chemical tongue" for the discrimination of short-length Aß peptides.
Mikrochim Acta
; 191(2): 95, 2024 01 15.
Article
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| MEDLINE
| ID: mdl-38224352
ABSTRACT
A "chemical tongue" is proposed based on thiomalic acid-capped quantum dots (QDs) with signal enrichment provided by excitation-emission matrix (EEM) fluorescence spectroscopy for the determination of close structural analogs-short-length amyloid ß (Aß) peptides related to Alzheimer's disease. Excellent discrimination is obtained by principal component analysis (PCA) for seven derivatives Aß1-16, Aß4-16, Aß4-9, Aß5-16, Aß5-12, Aß5-9, Aß12-16. Detection of Aß4-16, Aß4-16, and Aß5-9 in binary and ternary mixtures performed by QDs-based chemical tongue using partial least squares-discriminant analysis (PLS-DA) provided perfect 100% accuracy for the two studied peptides (Aß4-16 and Aß4-16), while for the third one (Aß5-9) it was slightly lower (97.9%). Successful detection of Aß4-16 at 1 pmol/mL (1.6 ng/mL) suggests that the detection limit of the proposed method for short-length Aß peptides can span nanomolar concentrations. This result is highly promising for the development of simple and efficient methods for sequence recognition in short-length peptides and better understanding of mechanisms at the QD-analyte interface.
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MEDLINE
Asunto principal:
Puntos Cuánticos
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Enfermedad de Alzheimer
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
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En
Revista:
Mikrochim Acta
Año:
2024
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Article