Efficacy of lysostaphin-coated titanium plates on implant-associated MRSA osteitis in minipigs.
Eur J Trauma Emerg Surg
; 50(3): 887-895, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38265442
ABSTRACT
PURPOSE:
The growing incidence of implant-associated infections (IAIs) caused by biofilm-forming Staphylococcus aureus in combination with an increasing resistance to antibiotics requires new therapeutic strategies. Lysostaphin has been shown to eliminate this biofilm. Own studies confirm the effectiveness in a murine model. The current study characterizes the effects of lysostaphin-coated plates in an IAI minipig model.METHODS:
The femur of 30 minipigs was stabilized with a five-hole plate, a bone defect was created, and in 20 cases methicillin-resistant Staphylococcus aureus was applied. Ten animals served as control group. After 14 days, local debridement, lavage, and plate exchange (seven-hole plate) were performed. Ten of the infected minipigs received an uncoated plate and 10 a lysostaphin-coated plate. On day 84, the minipigs were again lavaged, followed by euthanasia. Bacterial load was quantified by colony-forming units (CFU). Immunological response was determined by neutrophils, as well as interleukins. Fracture healing was assessed radiologically.RESULTS:
CFU showed significant difference between infected minipigs with an uncoated plate and minipigs with a lysostaphin-coated plate (p = 0.0411). The infection-related excessive callus formation and calcification was significantly greater in the infected animals with an uncoated plate than in animals with a lysostaphin-coated plate (p = 0.0164/p = 0.0033). The analysis of polymorphonuclear neutrophils and interleukins did not reveal any pioneering findings.CONCLUSION:
This study confirms the minipig model for examining IAI. Furthermore, coating of plates using lysostaphin could be a promising tool in the therapeutic strategies of IAI. Future studies should focus on coating technology of implants and on translation into a clinical model.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Osteítis
/
Infecciones Estafilocócicas
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Porcinos Enanos
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Placas Óseas
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Infecciones Relacionadas con Prótesis
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Modelos Animales de Enfermedad
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Staphylococcus aureus Resistente a Meticilina
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Lisostafina
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Eur J Trauma Emerg Surg
Año:
2024
Tipo del documento:
Article