Identification, chemical synthesis, and receptor binding of a reptilian gecko ghrelin.
J Pept Sci
; 30(6): e3567, 2024 Jun.
Article
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| MEDLINE
| ID: mdl-38268104
ABSTRACT
Ghrelin is known to be a gastrointestinal peptide hormone in vertebrates. It has a unique posttransrational modification, octanoylation, at the Ser side chain of the third position. In this study, we identified the genes encoding ghrelin and its receptor from the Schlegel's Japanese gecko Gekko japonicus. The C-terminal residue of gecko ghrelin was His, although the chemical synthesis method for the O-octanoyl peptide with a C-terminal His residue has not yet been well-established. Acyl-ghrelin has been synthesized using a Ser derivative without side chain protecting group in the solid-phase peptide synthesis, although this synthetic strategy has not yet been well-established. Here we show the efficient synthetic method with minimal side reactions, and G. japonicus ghrelin could be obtained in good yield. This would be useful and applicable to the synthesis of ghrelin from other animal species. The gecko ghrelin receptor was expressed in HEK 293 cells, which was fully responsive to the synthetic gecko ghrelin. These results indicate that the ghrelin system similar to mammals also exists in a reptilian gecko, G. japonicus.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Ghrelina
/
Receptores de Ghrelina
/
Lagartos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Pept Sci
Asunto de la revista:
BIOQUIMICA
Año:
2024
Tipo del documento:
Article