Noninvasive serum N-glycans associated with ovarian cancer diagnosis and precancerous lesion prediction.
J Ovarian Res
; 17(1): 26, 2024 Jan 27.
Article
en En
| MEDLINE
| ID: mdl-38281033
ABSTRACT
BACKGROUND:
Ovarian cancer (OC) is one of the most common gynecological tumors with high morbidity and mortality. Altered serum N-glycome has been observed in many diseases, while the association between serum protein N-glycosylation and OC progression remains unclear, particularly for the onset of carcinogenesis from benign neoplasms to cancer.METHODS:
Herein, a mass spectrometry based high-throughput technique was applied to characterize serum N-glycome profile in individuals with healthy controls, benign neoplasms and different stages of OC. To elucidate the alterations of glycan features in OC progression, an orthogonal strategy with lectin-based ELISA was performed.RESULTS:
It was observed that the initiation and development of OC was associated with increased high-mannosylationand agalactosylation, concurrently with decreased total sialylation of serum, each of which gained at least moderately accurate merits. The most important individual N-glycans in each glycan group was H7N2, H3N5 and H5N4S2F1, respectively. Notably, serum N-glycome could be used to accurately discriminate OC patients from benign cohorts, with a comparable or even higher diagnostic score compared to CA125 and HE4. Furthermore, bioinformatics analysis based discriminative model verified the diagnostic performance of serum N-glycome for OC in two independent sets.CONCLUSIONS:
These findings demonstrated the great potential of serum N-glycome for OC diagnosis and precancerous lesion prediction, paving a new way for OC screening and monitoring.Palabras clave
Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Neoplasias Ováricas
/
Lesiones Precancerosas
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
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Risk_factors_studies
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Screening_studies
Idioma:
En
Revista:
J Ovarian Res
Año:
2024
Tipo del documento:
Article