Colistin heteroresistance in Citrobacter freundii clinical isolates from Republic of Korea.
Diagn Microbiol Infect Dis
; 108(4): 116187, 2024 Apr.
Article
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| MEDLINE
| ID: mdl-38340485
ABSTRACT
We investigated colistin heteroresistance in Citrobacter freundii isolates from Korean hospitals. Using population analysis profiling (PAP), we detected colistin heteroresistance in 31.3% of isolates. Among these, ST217 was the most prevalent clone (58.5%), particularly within colistin-heteroresistant isolates (80.0%). Interestingly, the second most common clone, ST248, was not found in heteroresistant isolates. We identified amino acid changes in PhoQ, PmrA, and PmrB, along with mRNA overexpression in pmrB and arnD. Colistin monotherapy showed no efficacy, but a combination of colistin and ciprofloxacin successfully eradicated all five isolates, even at 0.5 × minimum inhibitory concentrations. This study underscores the high prevalence of colistin heteroresistance in C. freundii isolates, limiting the effectiveness of colistin monotherapy. Combining colistin with ciprofloxacin may offer a viable treatment option for C. freundii infections.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Colistina
/
Antibacterianos
Tipo de estudio:
Risk_factors_studies
País/Región como asunto:
Asia
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2024
Tipo del documento:
Article