A new chromosome-scale genome of wild Brassica oleracea provides insights into the domestication of Brassica crops.
J Exp Bot
; 75(10): 2882-2899, 2024 May 20.
Article
en En
| MEDLINE
| ID: mdl-38421062
ABSTRACT
The cultivated diploid Brassica oleracea is an important vegetable crop, but the genetic basis of its domestication remains largely unclear in the absence of high-quality reference genomes of wild B. oleracea. Here, we report the first chromosome-level assembly of the wild Brassica oleracea L. W03 genome (total genome size, 630.7 Mb; scaffold N50, 64.6 Mb). Using the newly assembled W03 genome, we constructed a gene-based B. oleracea pangenome and identified 29 744 core genes, 23 306 dispensable genes, and 1896 private genes. We re-sequenced 53 accessions, representing six potential wild B. oleracea progenitor species. The results of the population genomic analysis showed that the wild B. oleracea populations had the highest level of diversity and represents the most closely related population to modern-day horticultural B. oleracea. In addition, the WUSCHEL gene was found to play a decisive role in domestication and to be involved in cauliflower and broccoli curd formation. We also illustrate the loss of disease-resistance genes during selection for domestication. Our results provide new insights into the domestication of B. oleracea and will facilitate the future genetic improvement of Brassica crops.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Brassica
/
Genoma de Planta
/
Productos Agrícolas
/
Domesticación
Idioma:
En
Revista:
J Exp Bot
Asunto de la revista:
BOTANICA
Año:
2024
Tipo del documento:
Article