Comparative analysis of the diversity of symbionts in fat body of long- and short-winged brown planthoppers.
Arch Insect Biochem Physiol
; 115(3): e22096, 2024 Mar.
Article
en En
| MEDLINE
| ID: mdl-38500448
ABSTRACT
The microbial community structure plays an important role in the internal environment of brown planthopper (BPH), Nilaparvata lugens (Hemiptera Delphacidae), which is an indispensable part to reflect the internal environment of BPH. Wing dimorphism is a strategy for balancing flight and reproduction of insects. Here, quantitative fluorescence PCR was used to analyse the number and changes of the symbionts in the fat body of long- and short-winged BPHs at different developmental stages. A metagenomic library was constructed based on the 16 S rRNA sequence and internal transcribed spacer sequence for high-throughput sequencing, to analyze the community structure and population number of the symbionts of long- and short-winged BPHs, and to make functional prediction. This study enriches the connotation of BPH symbionts, and laid a theoretical foundation for the subsequent study of BPH-symbionts interaction and the function of symbionts in the host.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cuerpo Adiposo
/
Hemípteros
Idioma:
En
Revista:
Arch Insect Biochem Physiol
Asunto de la revista:
BIOLOGIA
/
BIOQUIMICA
Año:
2024
Tipo del documento:
Article